In-Vitro Evaluation of Antimicrobial Efficacy of Spherical Green Ag-Tio2 NPs Using Sajne Leaves Extricate of Moringa Oleifera in Aqueous Media

Aakash  Singh; Chandra  Kumar Dixit; Ruby Singh

doi:10.63053/ijset.67

Authors

Aakash Singh Department of Applied Sciences and Humanities, Institute of Engineering and Technology Dr Shakuntala Misra National Rehabilitation University, Lucknow, U.P. India Department of Chemistry
Chandra Kumar Dixit Department of Applied Sciences and Humanities, Institute of Engineering and Technology Dr Shakuntala Misra National Rehabilitation University, Lucknow, U.P. India Department of Chemistry
Ruby Singh Department of Chemistry, Jaipur National University, Jaipur Rajasthan, India

DOI:

https://doi.org/10.63053/ijset.67

Keywords:

Green synthesis, NPs, Moringa oleifera, Antibacterial activity, Antifungal activity

Abstract

Environmentally friendly and biocompatible methods were employed for the green synthesis of Ag-TiO2 nanoparticles (NPs) using the leaf extract of Moringa oleifera as a capping and reducing agent. The successful synthesis of Ag-TiO2 NPs was observed through a wavelength shift within one hour of reaction. The formation of nanoparticles was confirmed through multiple analytical techniques, including UV–VIS spectroscopy, FTIR, XRD, EDX, and SEM analysis. X-ray diffraction and electron microscopy confirmed the spherical structure and size of the nanoparticles. EDX analysis verified the presence of silver ions in the doped TiO2 lattice, while SEM revealed the spherical morphology of Ag-TiO2 NPs. Bacterial concentrations of 1.8 × 10⁸ CFU/ml were tested using 20 ml of three samples: Moringa oleifera leaf extract, TiO2 NPs, and Ag-TiO2 NPs at concentrations of 12.5 mg/L, 25 mg/L, and 50 mg/L, in three separate trials. The nanoparticles demonstrated significant antimicrobial activity against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli), as well as antifungal activity against Aspergillus niger (A. niger). These findings suggest that Ag-TiO2 NPs hold potential for applications in biomedical fields.

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